Membrane Transport in Living Systems

Pagliara Group

Pagliara's Group

Research Overview

Our research is focused on understanding how microbial cells interact with their environment both as a community and as individual cells with an emphasis of membrane transporter regulation. Besides standard microbiology techniques and omics approaches, we develop bespoke microfluidic devices that enable to accurately control the microenvironment surrounding the cells and perform a series of functional assays on the very same individual cell. This allows us to shed light on the physiology of rare subpopulations of cells that are generally hidden in ensemble measurements on clonal populations. We apply the technology we develop for collaborative studies on antimicrobial resistance, bacterial physiology, ageing in unicellular organisms and stem cell differentiation.

Recent publications

  1. M. Kellom, S. Pagliara, T. A. Richards, A. E. Santoro
    Exaggerated trans-membrane charge of ammonium transporters in nutrient-poor marine environments
    Open Biology 12, 220041 (2022) [Link][PDF]
  2. U. Łapińska, Ma. Voliotis, K. K. Lee, A. Campey, M. R. L. Stone, B. Tuck, W. Phetsang, B. Zhang, K. Tsaneva-Atanasova, M. A. T. Blaskovich, S. Pagliara
    Fast bacterial growth reduces antibiotic accumulation and efficacy
    eLife 11, e74062 (2022) [Link][PDF]
  3. G. Glover, M. Voliotis, U. Łapińska, B. M. Invergo, D. Soanes, P. O'Neill, K. Moore, N. Nikolic, P. G. Petrov, D. S. Milner, S. Roy, K. Heesom, T. A. Richards, K. Tsaneva-Atanasova, S. Pagliara
    Nutrient and salt depletion synergistically boosts glucose metabolism in individual Escherichia coli cells
    Communications Biology 5, 385 (2022) [Link][PDF]
  4. J. Cama, K. Al Nahas, M. Fletcher, K. Hammond, M. G. Ryadnov, U. F. Keyser, S. Pagliara
    An ultrasensitive microfluidic approach reveals correlations between the physico-chemical and biological activity of experimental peptide antibiotics
    Scientific Reports 12, 4005 (2022) [Link][PDF]
  5. T. Dimitriu, E. Kurilovich, U. Łapińska, K. Severinov, S. Pagliara, M. Szczelkun, E. Westra
    Bacteriostatic antibiotics promote CRISPR-Cas adaptive immunity by enabling increased spacer acquisition
    Cell Host and Microbe 30, 31 (2022) [Link][PDF]


  • 13-July-2022: New paper on membrane transport in the ocean out in Open Biology
  • 7-June-2022: Congratulations Ula! New paper out at eLife
  • 20-April-2022: Well done Georgie! New paper out at Communications Biology