Stefano Pagliara Peer-reviewed Publications
- E. L. Attrill, U. Łapińska, E. R. Westra, S. V. Harding, S. Pagliara
Slow growing bacteria survive bacteriophage in isolation
ISME Communications 3, 95 (2023) [Link][PDF]
- U. Łapińska, G. Glover, Z. Kahveci, N. A. T. Irwin, D. S. Milner, M. Tourte, S.-V. Albers, A. E. Santoro, T. A. Richards, S. Pagliara
Systematic comparison of unilamellar vesicles reveals that archaeal core lipid membranes are more permeable than bacterial membranes
PLoS Biology 21, e3002048 (2023) [Link][PDF]
- B. Zhang, W. Phetsang, M. R. L. Stone, S. Kc, M. S. Butler, M. A. Cooper, A. G. Elliott, U. Łapińska, M. Voliotis, K. Tsaneva-Atanasova, S. Pagliara, M. A. T. Blaskovich
Synthesis of vancomycin fluorescent probes that retain antimicrobial activity, identify Gram-positive bacteria, and detect Gram-negative outer membrane damage
Communications Biology 6, 409 (2023) [Link][PDF]
- J. L. Binsley, T. O. Myers, S. Pagliara, F. Y. Ogrin
Herringbone micromixers for particle filtration
Biomicrofluidics 17, 014106 (2023) [Link][PDF]
- Y. Zhang, I. Kepiro, M. G. Ryadnov, S. Pagliara
Single Cell Killing Kinetics Differentiate Phenotypic Bacterial Responses to Different Antibacterial Classes
Microbiology Spectrum 11, 03667 (2023) [Link][PDF]
- J. L. Binsley, S. Pagliara, F. Y Ogrin
Numerical investigation of flexible Purcell-like integrated microfluidic pumps
Journal of Applied Physics 132, 164701 (2022) [Link][PDF]
- M. Kellom, S. Pagliara, T. A. Richards, A. E. Santoro
Exaggerated trans-membrane charge of ammonium transporters in nutrient-poor marine environments
Open Biology 12, 220041 (2022) [Link][PDF]
- U. Łapińska, Ma. Voliotis, K. K. Lee, A. Campey, M. R. L. Stone, B. Tuck, W. Phetsang, B. Zhang, K. Tsaneva-Atanasova, M. A. T. Blaskovich, S. Pagliara
Fast bacterial growth reduces antibiotic accumulation and efficacy
eLife 11, e74062 (2022) [Link][PDF]
- G. Glover, M. Voliotis, U. Łapińska, B. M. Invergo, D. Soanes, P. O'Neill, K. Moore, N. Nikolic, P. G. Petrov, D. S. Milner, S. Roy, K. Heesom, T. A. Richards, K. Tsaneva-Atanasova, S. Pagliara
Nutrient and salt depletion synergistically boosts glucose metabolism in individual Escherichia coli cells
Communications Biology 5, 385 (2022) [Link][PDF]
- J. Cama, K. Al Nahas, M. Fletcher, K. Hammond, M. G. Ryadnov, U. F. Keyser, S. Pagliara
An ultrasensitive microfluidic approach reveals correlations between the physico-chemical and biological activity of experimental peptide antibiotics
Scientific Reports 12, 4005 (2022) [Link][PDF]
- T. Dimitriu, E. Kurilovich, U. Łapińska, K. Severinov, S. Pagliara, M. Szczelkun, E. Westra
Bacteriostatic antibiotics promote CRISPR-Cas adaptive immunity by enabling increased spacer acquisition
Cell Host and Microbe 30, 31 (2022) [Link][PDF]
- B. Zhang, M. R. L. Stone, K. C. Sanjaya, U. Łapińska, S. Pagliara, M. A. T. Blaskovich
Application of antibiotic-derived fluorescent probes to bacterial studies
Methods in Enzymology 665, 1 (2022) [Link][PDF]
- R. Conners, M. McLaren, U. Łapińska, K. Sanders, M. Stone, M. Blaskovich, S. Pagliara, B. Daum, J. Rakonjac, V. Gold
CryoEM structure of the outer membrane secretin channel pIV from the f1 filamentous bacteriophage
Nature Communications 12, 6316 (2021) [Link][PDF]
- E. Attrill, R. Claydon, U. Łapińska, M. Recker, S. Meaden, A. Brown, E. Westra, S. Harding, S. Pagliara
Individual bacteria in structured environments rely on phenotypic resistance to phage
PLoS Biology 19, e3001406 (2021) [Link][PDF]
- O. Goode, A. Smith, A. Zarkan, J. Cama, B. M. Invergo, D. Belgami, S. Cano-Muniz, J. Metz, P. O’Neill, A. Jeffries, I. H. Norville, J. David, D. Summers, S. Pagliara
Persister Escherichia coli Cells Have a Lower Intracellular pH than Susceptible Cells but Maintain Their pH in Response to Antibiotic Treatment
mBio 12, e00909 (2021) [Link][PDF]
- O. Goode, A. Smith, U. Łapińska, R. Bamford, Z. Kahveci, G. Glover, E. L. Attrill, A. Carr, J. Metz, S. Pagliara
Heterologous Protein Expression Favors the Formation of Protein Aggregates in Persister and Viable but Nonculturable Bacteria
ACS Infectious Diseases 7, 1848 (2021) [Link][PDF]
- K. Hammond, F. Cipcigan, K. Al Nahas, V. Losasso, H. Lewis, J. Cama, F. Martelli, P. W. Simcock, M. Fletcher, J. Ravi, P. J. Stansfeld, S. Pagliara, B. W. Hoogenboom, U. F. Keyser, M. S. P. Sansom, J. Crain, M. G. Ryadnov
Switching Cytolytic Nanopores into Antimicrobial Fractal Ruptures by a Single Side Chain Mutation
ACS Nano 15, 9679 (2021) [Link][PDF]
- R. Morrish, K. H. W. Yim, S. Pagliara, F. Palombo, R. Chahwan, N. Stone
Single cell label-free probing of chromatin dynamics during B lymphocyte maturation
Frontiers in cell and developmental biology, 9, 688 (2021) [Link][PDF]
- J. Cama, S. Pagliara
Microfluidic Single-Cell Phenotyping of the Activity of Peptide-Based Antimicrobials
Methods in Molecular Biology 2208, 237 (2021) [Link][PDF]
- F. Palombo, S. Pagliara, A. Singh, R. Chahwan
Probing the chromatin architecture
Frontiers in cell and developmental biology, 9, 727803 (2021) [Link][PDF]
- M. R. L. Stone, U. Łapińska, S. Pagliara, M. Masi, J. T. Blanchfield, M. A. T. Blaskovich
Fluorescent macrolide probes, synthesis and use in evaluation of bacterial resistance
RSC Chemical Biology 1, 395 (2020) [Link][PDF]
- J. L. Binsley, E. L. Martin, T. O. Myers, S. Pagliara, F. Y. Ogrin
Microfluidic devices powered by integrated elasto-magnetic pumps
Lab on a Chip DOI:10.1039/D0LC00935K (2020) [Link][PDF]
- J. Cama, M. Voliotis, J. Metz, A. Smith, J. Iannucci, U. F. Keyser, K. Tsaneva-Atanasova, S. Pagliara
Single-cell microfluidics facilitates the rapid quantification of antibiotic accumulation in Gram-negative bacteria
Lab on a Chip 20, 2765 (2020) [Link][PDF]
- B. Gironi, Z. Kahveci, B. McGill, B.-D. Lechner, S. Pagliara, J. Metz, A. Morresi, F. Palombo, P. Sassi, P. G. Petrov
Effect of DMSO on the mechanical and structural properties of model and biological membranes
Biophysical Journal 119, 274 (2020) [Link][PDF]
- M. B. Stie, M. Corezzi, A. D. J. Bombin, F. Ajalloueian, E. Attrill, S. Pagliara, J. Jacobsen, I. S. Chronakis, H. M. Nielsen, V. Foderà
Waterborne Electrospinning of Alpha-lactalbumin Generates Tunable and Biocompatible Nanofibers for Drug Delivery
ACS Applied Nano Materials 3, 1910 (2020) [Link][PDF]
- I. E. Kepiro, I. Marzuoli, K. Hammond, X. Ba, H. Lewis, M. Shaw, S. B. Gunnoo, E. De Santis, U. Łapińska, S. Pagliara, M. A. Holmes, C. D. Lorenz, B. W. Hoogenboom, F. Fraternali, M. G. Ryadnov
Engineering Chirally Blind Protein Pseudo-Capsids into Antibacterial Persisters
ACS Nano 14, 1609 (2020) [Link][PDF]
Coverage: The Times, The Telegraph, The Daily Mail
- U. Łapińska, G. Glover, P. Capilla-Lasheras, A. J. Young, S. Pagliara
Bacterial ageing in the absence of external stressors
Philosophical Transactions of the Royal Society B, 374, 20180442 (2019) [Link][PDF]
- T. A. Richards, R. Massana, S. Pagliara, N. Hall
Single cell ecology
Philosophical Transactions of the Royal Society B, 374, 20190076 (2019) [Link][PDF]
- A. Smith, J. Metz, S. Pagliara
MMHelper: An automated framework for the analysis of microscopy images acquired with the mother machine
Scientific reports, 9, 1 (2019) [Link][PDF]
- R. B. Morrish, M. Hermes, J. Metz, N. Stone, S. Pagliara, R. Chahwan, F. Palombo
Single cell imaging of nuclear architecture changes
Frontiers in cell and developmental biology, 7, 141 (2019) [Link][PDF]
- M. A. T. Blaskovich, W. Phetsang, M. R. L. Stone, U. Łapińska, S. Pagliara, R. Bhalla, M. A. Cooper
Antibiotic-derived molecular probes for bacterial imaging
Proceedings of SPIE, 10863, 1086303 (2019) [Link][PDF]
- E. L. Martin, M. T. Bryan, S. Pagliara, F. Y. Ogrin
Advanced processing of micropatterned elasto-magnetic membranes
IEEE Transactions on Magnetics, doi: 10.1109/TMAG.2018.2887347 (2019) [Link][PDF]
- A. Smith, A. Kaczmar, R. Bamford,
C. Smith, S. Frustaci, A. Kovacs-Simon, P. O'Neill, K. Moore, K. Paszkiewicz, R. W. Titball, S. Pagliara
The culture environment influences both gene regulation and phenotypic heterogeneity in Escherichia coli
Frontiers in Microbiology, 9, 1739 (2018) [Link][PDF]
- T. Lutz, A. H. Clowsley, R. Lin, S. Pagliara, L. Di Michele, C. Soeller
Versatile multiplexed super-resolution imaging of nanostructures by Quencher-Exchange-PAINT
Nano Research, doi.org/10.1007/s12274-018-1971-6 (2018) [Link][PDF]
- R. Bamford, A. Smith, J. Metz, G. Glover, R. W. Titball, S. Pagliara
Investigating the physiology of viable but non-culturable bacteria by microfluidics and time-lapse microscopy
BMC Biology, 15, 121, (2017) [Link][PDF]
Press Release: UoE
Coverage: Daily Mail, The Australian, Oxford Times, SBS, Medindia
- Y. Tan, J. Gladrow, U. F. Keyser, L. Dagdug, S Pagliara
Particle transport across a channel via an oscillating potential
Physical Review E, 96, 052401, (2017) [Link][PDF]
- A. C. Hodgson, C. M. Verstreken, C. L. Fisher,
U. F. Keyser, S. Pagliara, K. J. Chalut
A microfluidic device for characterizing nuclear
deformations
Lab on a Chip, 17, 805, (2017) [Link][PDF]
- J. Cama, M. Schaich, K. Al Nahas, S. Hernandez-Ainsa, S. Pagliara, U. F. Keyser
Optofluidic Measurement of the Lipid Permeability of Fluoroquinolones
Scientific Reports, 6, 32824, (2016) [Link][PDF]
- E. Locatelli, M. Pierno, F. Baldovin, E. Orlandini, Y. Tan, S. Pagliara
Single-file escape of colloidal particles from microfluidic channels
Physical Review Letters, 117, 038001, (2016) [Link][PDF]
- J. Cama, H. Bajaj, S. Pagliara, T. Maier, Y. Braun, M. Winterhalter, U. F. Keyser.
Quantification of fluoroquinolone uptake through the outer membrane channel OmpF of Escherichia coli
Journal of the American Chemical Society, 137,13836-13843, (2015) [Link][PDF]
- K. Misiunas, S. Pagliara, E. Lauga, J. R. Lister, U. F. Keyser.
Non-decaying hydrodynamic interactions along narrow channels.
Physical Review Letters, 115, 038301, (2015) [Link][PDF]
- O. Otto, P. Rosendahl, A. Mietke, S. Golfier, C. Herold, D. Klaue, S. Girardo, S. Pagliara, A. Ekpenyong, A. Jakobi, M. Wobus, N. T&opfner, U. F. Keyser, J. Mansfeld, E. Fischer-Friedrich, J. Guck.
Real-time deformability cytometry: on-the-fly cell mechanical phenotyping.
Nature Methods,12, 199-202, (2015)[Link][PDF]
Highly cited paper, top 1% in academic field
- S. Pagliara, S. L. Dettmer, K. Misiunas, L. Lea, Y. Tan, U. F. Keyser.
Diffusion coefficients and particle transport in synthetic membrane channels.
The European Physical Journal Special Topics , 223, 3145-3163, (2014).[Link] [PDF]
- S. Pagliara, S. L. Dettmer, U. F. Keyser
Channel-facilitated diffusion boosted by particle binding at the channel entrance.
Physical Review Letters 113, 048102, (2014). [Link] [PDF]
- K. D. Schleicher, S. L. Dettmer, L. E. Kapinos, S. Pagliara, U. F. Keyser, S. Jeney, R. Y.H. Lim
Selective Transport Control on Molecular Velcro Made From Intrinsically Disordered Proteins.
Nature Nanotechnology 9, 525, (2014). [Link] [PDF]
- S. L. Dettmer, S. Pagliara, K. Misiunas, U. F. Keyser
Anisotropic diffusion of spherical particles in closely confining microchannels..
Physical Review E 89, 062305, (2014). [Link] [PDF]
- J. Cama, C. Chimerel, S. Pagliara, A. Javer, U. F. Keyser
A Label-Free Microfluidic Assay to quantitatively study antibiotic diffusion through lipid membranes.
Lab on a Chip 14, 2303, (2014). [Link] [PDF]
- S. Pagliara, K. Franze, C. R. McClain, G. W. Wylde, C. L. Fisher, R. J. M. Franklin, A. J. Kabla., U. F. Keyser, K. J. Chalut
Auxetic nuclei in embryonic stem cells exiting pluripotency.
Nature Materials 13, 638, (2014). [Link] [PDF]
Highlighted in Science Editor's Choice
- S. L. Dettmer, U. F. Keyser, S. Pagliara
Local characterization of hindered Brownian motion by digital video microscopy and 3D particle tracking.
Review of Scientific Instruments 85, 023708, (2014). [Link] [PDF]
- A. Camposeo, I. Greenfeld, F. Tantussi, S. Pagliara, M. Moffa, F. Fuso, M. Allegrini, E. Zussman, D. Pisignano.
Local Mechanical Properties of Electrospun Fibers Correlate to Their Internal Nanostructure.
Nano Letters , 13, 5056-5062, (2013) [Link] [PDF]
- S. Pagliara, C. Schwall, U. F. Keyser.
Optimizing diffusive transport through a synthetic membrane channel.
Advanced Materials, 25, 844-849, (2013) [Link] [PDF]
- S. Pagliara, A. Polini, A. Camposeo, H. C. Schröder, W. E. G. Müller, D. Pisignano.
Electrical properties of in-vitro biomineralized recombinant silicatein deposited by microfluidics.
Applied Physics Letters, 101, 193702, (2012) [Link] [PDF]
- A. E. Ekpenyong, G. Whyte, K. Chalut, S. Pagliara, F. Lautenschläger, C. Fiddler, S. Paschke, U. F. Keyser, E. R. Chilvers, J. Guck
Viscoelastic Properties of Differentiating Blood Cells are Fate- and Function-Dependent.
PLoS One, 7, e45237, (2012) [Link] [PDF]
- V. Foderà, S. Pagliara, O. Otto, U. F. Keyser, A. M. Donald.
Microfluidics Reveals a Flow-Induced Large Scale Polymorphism of Protein Aggregates.
Journal of Physical Chemistry Letters, 3, 2803, (2012) [Link] [PDF]
- A. Polini, S. Pagliara, A. Camposeo, R. Cingolani, X. Wang, H. C. Schröder, W. E. G. Müller, D. Pisignano.
Optical properties of in-vitro biomineralised silica.
Scientific Reports, 2, 607, (2012) [Link] [PDF]
- S. Pagliara, C. Chimerel, D. G. A. L. Aarts, R. Langford, U. F. Keyser.
Colloid flow control in microchannels and detection by laser scattering.
Progress in colloid and polymer science, 139, 45, (2012).[Link] [PDF]
- C.-W. Lee, S. Pagliara, U. F. Keyser, J. J. Baumberg.
Perpendicular coupling to in-plane photonics using arc waveguides fabricated via two-photon polymerization.
Applied Physics Letters, 100, 171102,(2012). [Link] [PDF]
- S. Pagliara, C. Chimerel, D. G. A. L. Aarts, R. Langford, U. F. Keyser.
Parallel sub-micrometre channels with different dimensions for laser scattering detection.
Lab on a Chip, 11, 3365, (2011). [Link] [PDF]
- S. Pagliara, A. Camposeo, M. S. Vitiello, G. Scamarcio, R. Cingolani, D. Pisignano.
Optical anisotropy in single light-emitting polymer nanofibers.
Journal of Physical Chemistry C, 115, 20399, (2011). [Link] [PDF]
- A. Polini, S. Pagliara, A. Camposeo, A. Biasco, R. Cingolani, H. C. Schröder, W. E. G. Müller, D. Pisignano.
Biosilica electrically-insulating layers by soft lithography-assisted biomineralisation with recombinant silicatein.
Advanced Materials, 23, 4674, (2011). [Link] [PDF]
- D. Tu, S. Pagliara, A. Camposeo, G. Potente, E. Mele, R. Cingolani, D. Pisignano.
Soft nanolithography by polymer fibers.
Advanced Functional Materials, 21, 1040, (2011). [Link] [PDF]
- D. Tu, S. Pagliara, R. Cingolani, D. Pisignano.
An electrospun fiber phototransistor by the conjugated polymer MEH-PPV.
Applied Physics Letters, 98, 023307, (2011). [Link] [PDF]
- S. Pagliara, A. Camposeo, F. Di Benedetto, A. Polini, E. Mele, L. Persano, R. Cingolani, D. Pisignano.
Study of optical properties of electrospun light-emitting polymer nanofibers.
Superlattices and Microstructures,47, 145, (2010).[Link] [PDF]
- D. Tu, S. Pagliara, A. Camposeo, L. Persano, R. Cingolani, D. Pisignano.
Single light-emitting polymer nanofiber field-effect transistors.
Nanoscale, 2, 2217, (2010). [Link] [PDF]
- S. Pagliara, A. Camposeo, E. Mele, R. Cingolani, D. Pisignano.
Enhancement of light polarization from electrospun polymer fibers by room-temperature nanoimprint lithography.
Nanotechnology, 21, 215304, (2010). [Link] [PDF]
- A. Polini, S. Pagliara, R. Stabile, G. S. Netti, L. Roca, C. Prattichizzo, L. Gesualdo, R. Cingolani, D. Pisignano.
Collagen-functionalized Electrospun Polymer Nanofibers for Bioengineering Applications.
Soft Matter, 6, 1668, (2010). [Link] [PDF]
- A. A. R. Neves, A. Camposeo, S. Pagliara, R. Saija, F. Borghese, P. Denti, M. A. Iatì, R. Cingolani, O. M. Maragò, D. Pisignano.
Rotational dynamics of optically trapped polymeric nanofibers.
Optics Express, 18, 822, (2010). [Link] [PDF]
- S. Pagliara, A. Camposeo, R. Cingolani, D. Pisignano.
Hierarchical assembly of light-emitting polymer nanofibers in helical morphologies.
Applied Physics Letters, 95, 263301, (2009). [Link] [PDF]
- S. Pagliara, A. Camposeo, A. Polini, R. Cingolani, D. Pisignano.
Electrospun light-emitting nanofibers as excitation source in microfluidic devices.
Lab on a Chip, 9, 2851, (2009). [Link] [PDF]
- F. Di Benedetto, A. Camposeo, S. Pagliara, E. Mele, L. Persano, R. Stabile, R. Cingolani, D. Pisignano.
Patterning of light-emitting conjugated polymer nanofibres.
Nature Nanotechnology, 3, 614, (2008). [Link] [PDF]
- S. Pagliara, L. Persano, A. Camposeo, R. Cingolani, D. Pisignano.
Registration accuracy in multilevel soft lithography.
Nanotechnology, 18, 175302, (2007). [Link] [PDF]
News
- 8-September-2023: Congratulations Erin! New paper out at ISME Communications
- 10-April-2023: Congratulations Ula! New paper out at PLoS Biology
- 7-June-2022: Congratulations Ula! New paper out at eLife