Stefano Pagliara Peer-reviewed Publications

  1. E. L. Attrill, U. Łapińska, E. R. Westra, S. V. Harding, S. Pagliara
    Slow growing bacteria survive bacteriophage in isolation
    ISME Communications 3, 95 (2023) [Link][PDF]
  2. U. Łapińska, G. Glover, Z. Kahveci, N. A. T. Irwin, D. S. Milner, M. Tourte, S.-V. Albers, A. E. Santoro, T. A. Richards, S. Pagliara
    Systematic comparison of unilamellar vesicles reveals that archaeal core lipid membranes are more permeable than bacterial membranes
    PLoS Biology 21, e3002048 (2023) [Link][PDF]
  3. B. Zhang, W. Phetsang, M. R. L. Stone, S. Kc, M. S. Butler, M. A. Cooper, A. G. Elliott, U. Łapińska, M. Voliotis, K. Tsaneva-Atanasova, S. Pagliara, M. A. T. Blaskovich
    Synthesis of vancomycin fluorescent probes that retain antimicrobial activity, identify Gram-positive bacteria, and detect Gram-negative outer membrane damage
    Communications Biology 6, 409 (2023) [Link][PDF]
  4. J. L. Binsley, T. O. Myers, S. Pagliara, F. Y. Ogrin
    Herringbone micromixers for particle filtration
    Biomicrofluidics 17, 014106 (2023) [Link][PDF]
  5. Y. Zhang, I. Kepiro, M. G. Ryadnov, S. Pagliara
    Single Cell Killing Kinetics Differentiate Phenotypic Bacterial Responses to Different Antibacterial Classes
    Microbiology Spectrum 11, 03667 (2023) [Link][PDF]
  6. J. L. Binsley, S. Pagliara, F. Y Ogrin
    Numerical investigation of flexible Purcell-like integrated microfluidic pumps
    Journal of Applied Physics 132, 164701 (2022) [Link][PDF]
  7. M. Kellom, S. Pagliara, T. A. Richards, A. E. Santoro
    Exaggerated trans-membrane charge of ammonium transporters in nutrient-poor marine environments
    Open Biology 12, 220041 (2022) [Link][PDF]
  8. U. Łapińska, Ma. Voliotis, K. K. Lee, A. Campey, M. R. L. Stone, B. Tuck, W. Phetsang, B. Zhang, K. Tsaneva-Atanasova, M. A. T. Blaskovich, S. Pagliara
    Fast bacterial growth reduces antibiotic accumulation and efficacy
    eLife 11, e74062 (2022) [Link][PDF]
  9. G. Glover, M. Voliotis, U. Łapińska, B. M. Invergo, D. Soanes, P. O'Neill, K. Moore, N. Nikolic, P. G. Petrov, D. S. Milner, S. Roy, K. Heesom, T. A. Richards, K. Tsaneva-Atanasova, S. Pagliara
    Nutrient and salt depletion synergistically boosts glucose metabolism in individual Escherichia coli cells
    Communications Biology 5, 385 (2022) [Link][PDF]
  10. J. Cama, K. Al Nahas, M. Fletcher, K. Hammond, M. G. Ryadnov, U. F. Keyser, S. Pagliara
    An ultrasensitive microfluidic approach reveals correlations between the physico-chemical and biological activity of experimental peptide antibiotics
    Scientific Reports 12, 4005 (2022) [Link][PDF]
  11. T. Dimitriu, E. Kurilovich, U. Łapińska, K. Severinov, S. Pagliara, M. Szczelkun, E. Westra
    Bacteriostatic antibiotics promote CRISPR-Cas adaptive immunity by enabling increased spacer acquisition
    Cell Host and Microbe 30, 31 (2022) [Link][PDF]
  12. B. Zhang, M. R. L. Stone, K. C. Sanjaya, U. Łapińska, S. Pagliara, M. A. T. Blaskovich
    Application of antibiotic-derived fluorescent probes to bacterial studies
    Methods in Enzymology 665, 1 (2022) [Link][PDF]
  13. R. Conners, M. McLaren, U. Łapińska, K. Sanders, M. Stone, M. Blaskovich, S. Pagliara, B. Daum, J. Rakonjac, V. Gold
    CryoEM structure of the outer membrane secretin channel pIV from the f1 filamentous bacteriophage
    Nature Communications 12, 6316 (2021) [Link][PDF]
  14. E. Attrill, R. Claydon, U. Łapińska, M. Recker, S. Meaden, A. Brown, E. Westra, S. Harding, S. Pagliara
    Individual bacteria in structured environments rely on phenotypic resistance to phage
    PLoS Biology 19, e3001406 (2021) [Link][PDF]
  15. O. Goode, A. Smith, A. Zarkan, J. Cama, B. M. Invergo, D. Belgami, S. Cano-Muniz, J. Metz, P. O’Neill, A. Jeffries, I. H. Norville, J. David, D. Summers, S. Pagliara
    Persister Escherichia coli Cells Have a Lower Intracellular pH than Susceptible Cells but Maintain Their pH in Response to Antibiotic Treatment
    mBio 12, e00909 (2021) [Link][PDF]
  16. O. Goode, A. Smith, U. Łapińska, R. Bamford, Z. Kahveci, G. Glover, E. L. Attrill, A. Carr, J. Metz, S. Pagliara
    Heterologous Protein Expression Favors the Formation of Protein Aggregates in Persister and Viable but Nonculturable Bacteria
    ACS Infectious Diseases 7, 1848 (2021) [Link][PDF]
  17. K. Hammond, F. Cipcigan, K. Al Nahas, V. Losasso, H. Lewis, J. Cama, F. Martelli, P. W. Simcock, M. Fletcher, J. Ravi, P. J. Stansfeld, S. Pagliara, B. W. Hoogenboom, U. F. Keyser, M. S. P. Sansom, J. Crain, M. G. Ryadnov
    Switching Cytolytic Nanopores into Antimicrobial Fractal Ruptures by a Single Side Chain Mutation
    ACS Nano 15, 9679 (2021) [Link][PDF]
  18. R. Morrish, K. H. W. Yim, S. Pagliara, F. Palombo, R. Chahwan, N. Stone
    Single cell label-free probing of chromatin dynamics during B lymphocyte maturation
    Frontiers in cell and developmental biology, 9, 688 (2021) [Link][PDF]
  19. J. Cama, S. Pagliara
    Microfluidic Single-Cell Phenotyping of the Activity of Peptide-Based Antimicrobials
    Methods in Molecular Biology 2208, 237 (2021) [Link][PDF]
  20. F. Palombo, S. Pagliara, A. Singh, R. Chahwan
    Probing the chromatin architecture
    Frontiers in cell and developmental biology, 9, 727803 (2021) [Link][PDF]
  21. M. R. L. Stone, U. Łapińska, S. Pagliara, M. Masi, J. T. Blanchfield, M. A. T. Blaskovich
    Fluorescent macrolide probes, synthesis and use in evaluation of bacterial resistance
    RSC Chemical Biology 1, 395 (2020) [Link][PDF]
  22. J. L. Binsley, E. L. Martin, T. O. Myers, S. Pagliara, F. Y. Ogrin
    Microfluidic devices powered by integrated elasto-magnetic pumps
    Lab on a Chip DOI:10.1039/D0LC00935K (2020) [Link][PDF]
  23. J. Cama, M. Voliotis, J. Metz, A. Smith, J. Iannucci, U. F. Keyser, K. Tsaneva-Atanasova, S. Pagliara
    Single-cell microfluidics facilitates the rapid quantification of antibiotic accumulation in Gram-negative bacteria
    Lab on a Chip 20, 2765 (2020) [Link][PDF]
  24. B. Gironi, Z. Kahveci, B. McGill, B.-D. Lechner, S. Pagliara, J. Metz, A. Morresi, F. Palombo, P. Sassi, P. G. Petrov
    Effect of DMSO on the mechanical and structural properties of model and biological membranes
    Biophysical Journal 119, 274 (2020) [Link][PDF]
  25. M. B. Stie, M. Corezzi, A. D. J. Bombin, F. Ajalloueian, E. Attrill, S. Pagliara, J. Jacobsen, I. S. Chronakis, H. M. Nielsen, V. Foderà
    Waterborne Electrospinning of Alpha-lactalbumin Generates Tunable and Biocompatible Nanofibers for Drug Delivery
    ACS Applied Nano Materials 3, 1910 (2020) [Link][PDF]
  26. I. E. Kepiro, I. Marzuoli, K. Hammond, X. Ba, H. Lewis, M. Shaw, S. B. Gunnoo, E. De Santis, U. Łapińska, S. Pagliara, M. A. Holmes, C. D. Lorenz, B. W. Hoogenboom, F. Fraternali, M. G. Ryadnov
    Engineering Chirally Blind Protein Pseudo-Capsids into Antibacterial Persisters
    ACS Nano 14, 1609 (2020) [Link][PDF]
    Coverage: The Times, The Telegraph, The Daily Mail
  27. U. Łapińska, G. Glover, P. Capilla-Lasheras, A. J. Young, S. Pagliara
    Bacterial ageing in the absence of external stressors
    Philosophical Transactions of the Royal Society B, 374, 20180442 (2019) [Link][PDF]
  28. T. A. Richards, R. Massana, S. Pagliara, N. Hall
    Single cell ecology
    Philosophical Transactions of the Royal Society B, 374, 20190076 (2019) [Link][PDF]
  29. A. Smith, J. Metz, S. Pagliara
    MMHelper: An automated framework for the analysis of microscopy images acquired with the mother machine
    Scientific reports, 9, 1 (2019) [Link][PDF]
  30. R. B. Morrish, M. Hermes, J. Metz, N. Stone, S. Pagliara, R. Chahwan, F. Palombo
    Single cell imaging of nuclear architecture changes
    Frontiers in cell and developmental biology, 7, 141 (2019) [Link][PDF]
  31. M. A. T. Blaskovich, W. Phetsang, M. R. L. Stone, U. Łapińska, S. Pagliara, R. Bhalla, M. A. Cooper
    Antibiotic-derived molecular probes for bacterial imaging
    Proceedings of SPIE, 10863, 1086303 (2019) [Link][PDF]
  32. E. L. Martin, M. T. Bryan, S. Pagliara, F. Y. Ogrin
    Advanced processing of micropatterned elasto-magnetic membranes
    IEEE Transactions on Magnetics, doi: 10.1109/TMAG.2018.2887347 (2019) [Link][PDF]
  33. A. Smith, A. Kaczmar, R. Bamford, C. Smith, S. Frustaci, A. Kovacs-Simon, P. O'Neill, K. Moore, K. Paszkiewicz, R. W. Titball, S. Pagliara
    The culture environment influences both gene regulation and phenotypic heterogeneity in Escherichia coli
    Frontiers in Microbiology, 9, 1739 (2018) [Link][PDF]
  34. T. Lutz, A. H. Clowsley, R. Lin, S. Pagliara, L. Di Michele, C. Soeller
    Versatile multiplexed super-resolution imaging of nanostructures by Quencher-Exchange-PAINT
    Nano Research, (2018) [Link][PDF]
  35. R. Bamford, A. Smith, J. Metz, G. Glover, R. W. Titball, S. Pagliara
    Investigating the physiology of viable but non-culturable bacteria by microfluidics and time-lapse microscopy
    BMC Biology, 15, 121, (2017) [Link][PDF]
    Press Release: UoE
    Coverage: Daily Mail, The Australian, Oxford Times, SBS, Medindia
  36. Y. Tan, J. Gladrow, U. F. Keyser, L. Dagdug, S Pagliara
    Particle transport across a channel via an oscillating potential
    Physical Review E, 96, 052401, (2017) [Link][PDF]
  37. A. C. Hodgson, C. M. Verstreken, C. L. Fisher, U. F. Keyser, S. Pagliara, K. J. Chalut
    A microfluidic device for characterizing nuclear deformations
    Lab on a Chip, 17, 805, (2017) [Link][PDF]
  38. J. Cama, M. Schaich, K. Al Nahas, S. Hernandez-Ainsa, S. Pagliara, U. F. Keyser
    Optofluidic Measurement of the Lipid Permeability of Fluoroquinolones
    Scientific Reports, 6, 32824, (2016) [Link][PDF]
  39. E. Locatelli, M. Pierno, F. Baldovin, E. Orlandini, Y. Tan, S. Pagliara
    Single-file escape of colloidal particles from microfluidic channels
    Physical Review Letters, 117, 038001, (2016) [Link][PDF]
  40. J. Cama, H. Bajaj, S. Pagliara, T. Maier, Y. Braun, M. Winterhalter, U. F. Keyser.
    Quantification of fluoroquinolone uptake through the outer membrane channel OmpF of Escherichia coli
    Journal of the American Chemical Society, 137,13836-13843, (2015) [Link][PDF]
  41. K. Misiunas, S. Pagliara, E. Lauga, J. R. Lister, U. F. Keyser.
    Non-decaying hydrodynamic interactions along narrow channels.
    Physical Review Letters, 115, 038301, (2015) [Link][PDF]
  42. O. Otto, P. Rosendahl, A. Mietke, S. Golfier, C. Herold, D. Klaue, S. Girardo, S. Pagliara, A. Ekpenyong, A. Jakobi, M. Wobus, N. T&opfner, U. F. Keyser, J. Mansfeld, E. Fischer-Friedrich, J. Guck.
    Real-time deformability cytometry: on-the-fly cell mechanical phenotyping.
    Nature Methods,12, 199-202, (2015)[Link][PDF]
    Highly cited paper, top 1% in academic field
  43. S. Pagliara, S. L. Dettmer, K. Misiunas, L. Lea, Y. Tan, U. F. Keyser.
    Diffusion coefficients and particle transport in synthetic membrane channels.
    The European Physical Journal Special Topics , 223, 3145-3163, (2014).[Link] [PDF]
  44. S. Pagliara, S. L. Dettmer, U. F. Keyser
    Channel-facilitated diffusion boosted by particle binding at the channel entrance.
    Physical Review Letters 113, 048102, (2014). [Link] [PDF]
  45. K. D. Schleicher, S. L. Dettmer, L. E. Kapinos, S. Pagliara, U. F. Keyser, S. Jeney, R. Y.H. Lim
    Selective Transport Control on Molecular Velcro Made From Intrinsically Disordered Proteins.
    Nature Nanotechnology 9, 525, (2014). [Link] [PDF]
  46. S. L. Dettmer, S. Pagliara, K. Misiunas, U. F. Keyser
    Anisotropic diffusion of spherical particles in closely confining microchannels..
    Physical Review E 89, 062305, (2014). [Link] [PDF]
  47. J. Cama, C. Chimerel, S. Pagliara, A. Javer, U. F. Keyser
    A Label-Free Microfluidic Assay to quantitatively study antibiotic diffusion through lipid membranes.
    Lab on a Chip 14, 2303, (2014). [Link] [PDF]
  48. S. Pagliara, K. Franze, C. R. McClain, G. W. Wylde, C. L. Fisher, R. J. M. Franklin, A. J. Kabla., U. F. Keyser, K. J. Chalut
    Auxetic nuclei in embryonic stem cells exiting pluripotency.
    Nature Materials 13, 638, (2014). [Link] [PDF]
    Highlighted in Science Editor's Choice
  49. S. L. Dettmer, U. F. Keyser, S. Pagliara
    Local characterization of hindered Brownian motion by digital video microscopy and 3D particle tracking.
    Review of Scientific Instruments 85, 023708, (2014). [Link] [PDF]
  50. A. Camposeo, I. Greenfeld, F. Tantussi, S. Pagliara, M. Moffa, F. Fuso, M. Allegrini, E. Zussman, D. Pisignano.
    Local Mechanical Properties of Electrospun Fibers Correlate to Their Internal Nanostructure.
    Nano Letters , 13, 5056-5062, (2013) [Link] [PDF]
  51. S. Pagliara, C. Schwall, U. F. Keyser.
    Optimizing diffusive transport through a synthetic membrane channel.
    Advanced Materials, 25, 844-849, (2013) [Link] [PDF]
  52. S. Pagliara, A. Polini, A. Camposeo, H. C. Schröder, W. E. G. Müller, D. Pisignano.
    Electrical properties of in-vitro biomineralized recombinant silicatein deposited by microfluidics.
    Applied Physics Letters, 101, 193702, (2012) [Link] [PDF]
  53. A. E. Ekpenyong, G. Whyte, K. Chalut, S. Pagliara, F. Lautenschläger, C. Fiddler, S. Paschke, U. F. Keyser, E. R. Chilvers, J. Guck
    Viscoelastic Properties of Differentiating Blood Cells are Fate- and Function-Dependent.
    PLoS One, 7, e45237, (2012) [Link] [PDF]
  54. V. Foderà, S. Pagliara, O. Otto, U. F. Keyser, A. M. Donald.
    Microfluidics Reveals a Flow-Induced Large Scale Polymorphism of Protein Aggregates.
    Journal of Physical Chemistry Letters, 3, 2803, (2012) [Link] [PDF]
  55. A. Polini, S. Pagliara, A. Camposeo, R. Cingolani, X. Wang, H. C. Schröder, W. E. G. Müller, D. Pisignano.
    Optical properties of in-vitro biomineralised silica.
    Scientific Reports, 2, 607, (2012) [Link] [PDF]
  56. S. Pagliara, C. Chimerel, D. G. A. L. Aarts, R. Langford, U. F. Keyser.
    Colloid flow control in microchannels and detection by laser scattering.
    Progress in colloid and polymer science, 139, 45, (2012).[Link] [PDF]
  57. C.-W. Lee, S. Pagliara, U. F. Keyser, J. J. Baumberg.
    Perpendicular coupling to in-plane photonics using arc waveguides fabricated via two-photon polymerization.
    Applied Physics Letters, 100, 171102,(2012). [Link] [PDF]
  58. S. Pagliara, C. Chimerel, D. G. A. L. Aarts, R. Langford, U. F. Keyser.
    Parallel sub-micrometre channels with different dimensions for laser scattering detection.
    Lab on a Chip, 11, 3365, (2011). [Link] [PDF]
  59. S. Pagliara, A. Camposeo, M. S. Vitiello, G. Scamarcio, R. Cingolani, D. Pisignano.
    Optical anisotropy in single light-emitting polymer nanofibers.
    Journal of Physical Chemistry C, 115, 20399, (2011). [Link] [PDF]
  60. A. Polini, S. Pagliara, A. Camposeo, A. Biasco, R. Cingolani, H. C. Schröder, W. E. G. Müller, D. Pisignano.
    Biosilica electrically-insulating layers by soft lithography-assisted biomineralisation with recombinant silicatein.
    Advanced Materials, 23, 4674, (2011). [Link] [PDF]
  61. D. Tu, S. Pagliara, A. Camposeo, G. Potente, E. Mele, R. Cingolani, D. Pisignano.
    Soft nanolithography by polymer fibers.
    Advanced Functional Materials, 21, 1040, (2011). [Link] [PDF]
  62. D. Tu, S. Pagliara, R. Cingolani, D. Pisignano.
    An electrospun fiber phototransistor by the conjugated polymer MEH-PPV.
    Applied Physics Letters, 98, 023307, (2011). [Link] [PDF]
  63. S. Pagliara, A. Camposeo, F. Di Benedetto, A. Polini, E. Mele, L. Persano, R. Cingolani, D. Pisignano.
    Study of optical properties of electrospun light-emitting polymer nanofibers.
    Superlattices and Microstructures,47, 145, (2010).[Link] [PDF]
  64. D. Tu, S. Pagliara, A. Camposeo, L. Persano, R. Cingolani, D. Pisignano.
    Single light-emitting polymer nanofiber field-effect transistors.
    Nanoscale, 2, 2217, (2010). [Link] [PDF]
  65. S. Pagliara, A. Camposeo, E. Mele, R. Cingolani, D. Pisignano.
    Enhancement of light polarization from electrospun polymer fibers by room-temperature nanoimprint lithography.
    Nanotechnology, 21, 215304, (2010). [Link] [PDF]
  66. A. Polini, S. Pagliara, R. Stabile, G. S. Netti, L. Roca, C. Prattichizzo, L. Gesualdo, R. Cingolani, D. Pisignano.
    Collagen-functionalized Electrospun Polymer Nanofibers for Bioengineering Applications.
    Soft Matter, 6, 1668, (2010). [Link] [PDF]
  67. A. A. R. Neves, A. Camposeo, S. Pagliara, R. Saija, F. Borghese, P. Denti, M. A. Iatì, R. Cingolani, O. M. Maragò, D. Pisignano.
    Rotational dynamics of optically trapped polymeric nanofibers.
    Optics Express, 18, 822, (2010). [Link] [PDF]
  68. S. Pagliara, A. Camposeo, R. Cingolani, D. Pisignano.
    Hierarchical assembly of light-emitting polymer nanofibers in helical morphologies.
    Applied Physics Letters, 95, 263301, (2009). [Link] [PDF]
  69. S. Pagliara, A. Camposeo, A. Polini, R. Cingolani, D. Pisignano.
    Electrospun light-emitting nanofibers as excitation source in microfluidic devices.
    Lab on a Chip, 9, 2851, (2009). [Link] [PDF]
  70. F. Di Benedetto, A. Camposeo, S. Pagliara, E. Mele, L. Persano, R. Stabile, R. Cingolani, D. Pisignano.
    Patterning of light-emitting conjugated polymer nanofibres.
    Nature Nanotechnology, 3, 614, (2008). [Link] [PDF]
  71. S. Pagliara, L. Persano, A. Camposeo, R. Cingolani, D. Pisignano.
    Registration accuracy in multilevel soft lithography.
    Nanotechnology, 18, 175302, (2007). [Link] [PDF]


  • 8-September-2023: Congratulations Erin! New paper out at ISME Communications
  • 10-April-2023: Congratulations Ula! New paper out at PLoS Biology
  • 7-June-2022: Congratulations Ula! New paper out at eLife