Membrane Transport in Living Systems

Pagliara Group

Pagliara's Group

Research Overview

Our research is focused on understanding how microbial cells interact with their environment both as a community and as individual cells with an emphasis of membrane transporter regulation. Besides standard microbiology techniques and omics approaches, we develop bespoke microfluidic devices that enable to accurately control the microenvironment surrounding the cells and perform a series of functional assays on the very same individual cell. This allows us to shed light on the physiology of rare subpopulations of cells that are generally hidden in ensemble measurements on clonal populations. We apply the technology we develop for collaborative studies on antimicrobial resistance, bacterial physiology, ageing in unicellular organisms and stem cell differentiation.

Recent publications

  1. T. Dimitriu, E. Kurilovich, U. Łapińska, K. Severinov, S. Pagliara, M. Szczelkun, E. Westra
    Bacteriostatic antibiotics promote CRISPR-Cas adaptive immunity by enabling increased spacer acquisition
    Cell Host and Microbe 30, 31 (2022) [Link][PDF]
  2. R. Conners, M. McLaren, U. Łapińska, K. Sanders, M. Stone, M. Blaskovich, S. Pagliara, B. Daum, J. Rakonjac, V. Gold
    CryoEM structure of the outer membrane secretin channel pIV from the f1 filamentous bacteriophage
    Nature Communications 12, 6316 (2021) [Link][PDF]
  3. E. Attrill, R. Claydon, U. Łapińska, M. Recker, S. Meaden, A. Brown, E. Westra, S. Harding, S. Pagliara
    Individual bacteria in structured environments rely on phenotypic resistance to phage
    PLoS Biology 19, e3001406 (2021) [Link][PDF]
  4. O. Goode, A. Smith, A. Zarkan, J. Cama, B. M. Invergo, D. Belgami, S. Cano-Muniz, J. Metz, P. O’Neill, A. Jeffries, I. H. Norville, J. David, D. Summers, S. Pagliara
    Persister Escherichia coli Cells Have a Lower Intracellular pH than Susceptible Cells but Maintain Their pH in Response to Antibiotic Treatment
    mBio 12, e00909 (2021) [Link][PDF]
  5. O. Goode, A. Smith, U. Łapińska,, R. Bamford, Z. Kahveci, G. Glover, E. L. Attrill, A. Carr, J. Metz, S. Pagliara
    Heterologous Protein Expression Favors the Formation of Protein Aggregates in Persister and Viable but Nonculturable Bacteria
    ACS Infectious Diseases 7, 1848 (2021) [Link][PDF]
  6. K. Hammond, F. Cipcigan, K. Al Nahas, V. Losasso, H. Lewis, J. Cama, F. Martelli, P. W. Simcock, M. Fletcher, J. Ravi, P. J. Stansfeld, S. Pagliara, B. W. Hoogenboom, U. F. Keyser, M. S. P. Sansom, J. Crain, M. G. Ryadnov
    Switching Cytolytic Nanopores into Antimicrobial Fractal Ruptures by a Single Side Chain Mutation
    ACS Nano 15, 9679 (2021) [Link][PDF]

News

  • 23-Oct-2020: Well done Jake, new integrated micropumps on Lab Chip
  • 21-Sep-2020: Welcome Ka Kiu joining our efforts to disclose the mechanisms underlying antibiotic accumulation in bacteria
  • 15-Jun-2020: Our latest work on antimicrobial resistance in Science Daily