Research Overview
Our research is focused on understanding how microbial cells interact with their environment both as a community and as individual cells with an emphasis of membrane transporter regulation. Besides standard techniques such as CFU assays, flow cytometry, mass spectrometry, real-time quantitative PCR and RNA sequencing, we develop bespoke microfluidic devices that enable to accurately control the microenvironment surrounding the cells and perform a series of functional assays on the very same individual cell. This allows us to shed light on the physiology of rare subpopulations of cells that are generally hidden in ensemble measurements on clonal populations. Furthermore, we apply the technology we develop for collaborative studies on microbes, mammalian, plant, algal cells and synthetic systems such as giant unilammellar vesicles and colloidal particles.
Recent publications
- I. E. Kepiro, I. Marzuoli, K. Hammond, X. Ba, H. Lewis, M. Shaw, S. B. Gunnoo, E. De Santis, U. Łapińska, S. Pagliara, M. A. Holmes, C. D. Lorenz, B. W. Hoogenboom, F. Fraternali, M. G. Ryadnov
Engineering Chirally Blind Protein Pseudo-Capsids into Antibacterial Persisters
ACS Nano (2019) [Link][PDF]
- U. Łapińska, G. Glover, P. Capilla-Lasheras, A. J. Young, S. Pagliara
Bacterial ageing in the absence of external stressors
Philosophical Transactions of the Royal Society B, 374, 20180442 (2019) [Link][PDF]
- T. A. Richards, R. Massana, S. Pagliara, N. Hall
Single cell ecology
Philosophical Transactions of the Royal Society B, 374, 20190076 (2019) [Link][PDF]
- A. Smith, J. Metz, S. Pagliara
MMHelper: An automated framework for the analysis of microscopy images acquired with the mother machine
Scientific reports, 9, 1 (2019) [Link][PDF]
- R. B. Morrish, M. Hermes, J. Metz, N. Stone, S. Pagliara, R. Chahwan, F. Palombo
Single cell imaging of nuclear architecture changes
Frontiers in cell and developmental biology, 7, 141 (2019) [Link][PDF]
