Membrane Transport in Living Systems

Pagliara Group

Tweets by @PagliaraExeter

Research Overview

Our research is focused on understanding how microbial cells interact with their environment both as a community and as individual cells with an emphasis of membrane transporter regulation. Besides standard microbiology techniques and omics approaches, we develop bespoke microfluidic devices that enable to accurately control the microenvironment surrounding the cells and perform a series of functional assays on the very same individual cell. This allows us to shed light on the physiology of rare subpopulations of cells that are generally hidden in ensemble measurements on clonal populations. We apply the technology we develop for collaborative studies on antimicrobial resistance, bacterial physiology, ageing in unicellular organisms and stem cell differentiation.

Recent publications

1. M. B. Stie, M. Corezzi, A. D. J. Bombin, F. Ajalloueian, E. Attrill, S. Pagliara, J. Jacobsen, I. S. Chronakis, H. M. Nielsen, V. Foderà
   

   Waterborne Electrospinning of Alpha-lactalbumin Generates Tunable and Biocompatible Nanofibers for Drug Delivery

   ACS Applied Nano Materials 3, 1910 (2020) [Link][PDF]
   
2. I. E. Kepiro, I. Marzuoli, K. Hammond, X. Ba, H. Lewis, M. Shaw, S. B. Gunnoo, E. De Santis, U. Łapińska, S. Pagliara, M. A. Holmes, C. D. Lorenz, B. W. Hoogenboom, F. Fraternali, M. G. Ryadnov
   

   Engineering Chirally Blind Protein Pseudo-Capsids into Antibacterial Persisters

   ACS Nano (2020) [Link][PDF]
   
3. U. Łapińska, G. Glover, P. Capilla-Lasheras, A. J. Young, S. Pagliara
   

   Bacterial ageing in the absence of external stressors

   Philosophical Transactions of the Royal Society B, 374, 20180442 (2019) [Link][PDF]
   
4. T. A. Richards, R. Massana, S. Pagliara, N. Hall
   

   Single cell ecology

   Philosophical Transactions of the Royal Society B, 374, 20190076 (2019) [Link][PDF]
   
5. A. Smith, J. Metz, S. Pagliara
   

   MMHelper: An automated framework for the analysis of microscopy images acquired with the mother machine

   Scientific reports, 9, 1 (2019) [Link][PDF]
   
6. R. B. Morrish, M. Hermes, J. Metz, N. Stone, S. Pagliara, R. Chahwan, F. Palombo
   

   Single cell imaging of nuclear architecture changes

   Frontiers in cell and developmental biology, 7, 141 (2019) [Link][PDF]