Our research is focused on understanding how microbial cells interact with their environment both as a community and as individual cells with an emphasis of membrane transporter regulation. Besides standard microbiology techniques and omics approaches, we develop bespoke microfluidic devices that enable to accurately control the microenvironment surrounding the cells and perform a series of functional assays on the very same individual cell. This allows us to shed light on the physiology of rare subpopulations of cells that are generally hidden in ensemble measurements on clonal populations. We apply the technology we develop for collaborative studies on antimicrobial resistance, bacterial physiology, ageing in unicellular organisms and stem cell differentiation.
Recent publications
T. Dimitriu, E. Kurilovich, U. Łapińska, K. Severinov, S. Pagliara, M. Szczelkun, E. Westra
O. Goode, A. Smith, A. Zarkan, J. Cama, B. M. Invergo, D. Belgami, S. Cano-Muniz, J. Metz, P. O’Neill, A. Jeffries, I. H. Norville, J. David, D. Summers, S. Pagliara
Persister Escherichia coli Cells Have a Lower Intracellular pH than Susceptible Cells but Maintain Their pH in Response to Antibiotic Treatment
K. Hammond, F. Cipcigan, K. Al Nahas, V. Losasso, H. Lewis, J. Cama, F. Martelli, P. W. Simcock, M. Fletcher, J. Ravi, P. J. Stansfeld, S. Pagliara, B. W. Hoogenboom, U. F. Keyser, M. S. P. Sansom, J. Crain, M. G. Ryadnov
Switching Cytolytic Nanopores into Antimicrobial Fractal Ruptures by a Single Side Chain Mutation