P2.1: Manganese Catalase and Superoxide Dismutase from
the Facultatively Aerobic Hyperthermophile, Pyrobaculum calidifontis
Amo, T.; Atomi, H.; Imanaka, T.
Department of Synthetic Chemistry and Biological
Chemistry, Graduate School of Engineering,
Kyoto University, Japan, Katsura, Nishikyo-ku, Kyoto 615-8510
P2.2: Preliminary Crystallographic Analysis of ATP-Dependent
Phosphofructokinase/Sugar Kinase from Methanococcus jannaschii
Arnfors, L. M. V.1; Hansen, T.2; Schönheit, P.2;
Meining, W.1,3; Ladenstein, R.1
1 Department of Biosciences, Karolinska Institutet,
Sweden
2 Institut für Allgemeine Mikrobiologie, Christian-Albrechts-Universität
Kiel, Germany
3 Section for Natural Sciences, Södertörns Högskola,
Sweden
P2.3: Low-Molecular Weight Compounds from Thermophiles
in Protein Stabilization Applications
Bagyan, I; Lentzen, G; Schwarz, T
Bitop AG, Stockumer Strasse 28, 548453 Witten,
Germany
P2.4: Exploring the Potential of Hex1 as a Vehicle for
Foreign Protein Expression in Filamentous Fungi Such as Trichoderma
reesei
Bergquist, P. L. 1,2,3; Curach, N. C.1,2; Te'o, V. S.
J.1,2; Gibbs, M. D.1,2; Nevalainen, K. M. H.1,2
1 Department of Biological Sciences, Macquarie
University, Sydney, NSW 2109, Australia;
2 Macquarie University Biotechnology Research Institute, Sydney,
NSW 2109, Australia;
3 Department of Molecular Medicine & Pathology, Auckland
University Medical School, Auckland, New Zealand.
P2.5: Enhanced Expression of a Thermophilic Bacterial
Xylanase (XynB) In Trichoderma reesei
Bergquist, P. L.1,2,3 ; Te'o, V. S. J. 1,2; Nevalainen,
K. M. H 1,2.
1Department of Biological Sciences, Macquarie
University, Sydney, NSW, Australia;
2Research Institute for Biotechnology, Macquarie University,
Sydney, NSW, Australia;
3Department of Molecular Medicine & Pathology, University
of Auckland Medical School, Auckland New Zealand
P2.6: Mining of the Genome of the (Hyper) Thermophilic
Micro-Organism Thermotoga maritima MSB8 for Enzymes which
are Active on Starch
Butler, D. 1,2, Eeuwema, W1,3, Dijkhuizen, L.1,3, van
der Maarel, M.J.E.C.1,2
1. Centre for Carbohydrate Bioengineering TNO-RUG
P.O. Box 14, 9750 AA Haren, The Netherlands
2. Innovative Ingredients and Products Department, TNO Nutrition
and Food Research, Rouaanstraat 27, 9723 CC, Groningen, The
Netherlands.
3. Microbial Physiology Research Group, Groningen Biomolecular
Sciences and Biotechnology Institute (GBB), University of Groningen,
Kerklaan 30, 9751 NN, Haren, The Netherlands
P2.7: Enzyme Engineering of a Thermostable Nitrile Hydratase
Cameron, R and Cowan, D
Advanced Research Centre for Applied Microbiology,
Department of Biotechnology, University of the Western Cape,
Cape Town, South Africa
P2.8: A GDP-Mannose Pyrophosphorylase Gene from Sulfolobus
solfataricus: Characterization in vivo and Expression in
E. coli
Sacchetti, S., Bartolucci, S. and * Cannio, R.
Dipartimento di Chimica Biologica, Università
degli Studi di Napoli "Federico II", Via Mezzocannone,
16, 80134, Napoli, Italy
*Istituto di Biochimica delle Proteine, CNR, Via P. Castellino,
111, 80131, Napoli, Italy
P2.9: Studies of the thermophilic Pyrrolidone Carboxyl
Peptidase from Thermococcus litoralis
Connelly, S.; Line, K; Isupov M,N
Biocatalysis Centre, Department of Biological
and Chemical Sciences, University of Exeter, UK
P2.10: A Thermophilic Enzyme from Corallina officinalis
Coupe, E.E.; Garcia-Rodriguez, E.; Littlechild, J.A.
Exeter Biocatalysis Centre, School of Biological
and Chemical Sciences, University of Exeter, UK
P2.11: Recombinant Thermostable AP Exonuclease from
Thermoanaerobacter tengcongensis: Cloning, Expression,
Purification, Properties and PCR Application
Dabrowski, S.; Ahring, B. K.
The Environmental Microbiology & Biotechnology
Research Group, BioCentrum, The Technical University of Denmark,
Søltofts Plads, Building 227, DK-2800 Kgs. Lyngby, Denmark
P2.12: Thermophilic Enzymatic Hydrolysis of Aldoximes
and Nitriles Using Microbial Inocula from Maize Silage
De Silva, DS1; Taylor, JP1; Kelly, DR2; King, D2; Knowles,
CJ3; Baker, SC1.
1 School of Biological and Chemical Sciences,
Birkbeck College, University of, London, Malet Street, London,
WC1E 7HX
2 Department of Chemistry, Cardiff University, PO Box 912, Cardiff,
CF10 3TB
3 Oxford Centre for Environmental Biotechnology, University
of Oxford, 43 Banbury Road, Oxford, OX2 6PE
P2.13: Comparative Study on Arabinose Isomerase (Galactose
Isomerase) from Bacillus halodurans, Geobacillus stearothermophilus,
and Thermotoga maritima
Lee, D-W. ; Choe, E-A; Lee, S-J; Hong, Y-H; Kim, S-B;
Lee, D-Y; Pyun, Y-R
Department of Biotechnology and Bioproducts Research
Center, Yonsei University, Seoul 120-749, Korea.
P2.14: Characterization of the Hypothetical Protein
(TM0416) from Thermotoga maritima: Bioconversion Activity
of D-Tagatose to D-Sorbose
Lee, D-Y.; Lee, D-W; Lee, S-J; Choe, E-A; Hong, Y-H;
Kim, S-B; Pyun, Y-R
Department of Biotechnology and Bioproducts Reaserch
Center, Yonsei University, Seoul 120-749, Korea.
P2.15: Site-Directed Mutagenesis to Invert Coenzyme
Specificity in Glucose Dehydrogenase from Haloferax mediterranei
Ferrer, J.; Esclapez, J.; Pire, C.; Bonete, M.J.
Department of Biochemistry and Molecular Biology.
University of Alicante. Alicante. Spain
P2.16: Hydrogenases and Ferredoxins from Aquifex
aeolicus: Biochemical, Biophysical and Phylogenetic Characterization
Giudici-Orticoni, M.T., Tron, P., Bauzan, M., Bruschi,
M., Nitschke, W
Laboratoire de Bioénergétique et
Ingénierie des Protéines, CNRS - IBSM, 31 Chemin
Joseph Aiguier 13402 Marseille cedex 20, France
P2.17: Heat and Urea Induced Activations by Subunit
Rearrangement of Hyperthermophilic Glutamate Dehydrogenase Produced
in Escherichia coli
Goda, S1; Sakuraba, H1; Bhuiya, MW1; Kawakami, R1; Kuramitsu,
S2; Hiragi, Y3,4; Ohshima T1
1Department of Biological Science and Technology,
Faculty of Engineering, The University of Tokushima, Japan
2Department of Biology, Graduate School of Science, Osaka University,
Japan
3Institute for Chemical Research, Kyoto University, Japan
4Present address: Department of Physics, Kansai Medical University,
Japan
P2.18: Current Knowledge About the Biosynthesis of Glycogen
in Thermococcus hydrothermalis
Gruyer, S1 ; Legin, E1 ; Givry, S1 ; Bliard, C2 ; Duchiron,
F1
1 : Laboratoire de microbiologie industrielle,
UMR FARE, Université de Reims Champagne Ardenne, UFR
Sciences, Moulin de la Housse, BP 1039 Reims cedex 2, France
2 : Laboratoire de Pharmacognosie, CNRS, Université de
Reims Champagne Ardenne, UFR Sciences, Moulin de la Housse,
BP 1039 Reims cedex 2, France
P2.19: The Structure of a Hyperthermophilic Alcohol
Dehydrogenase
Guy, J.E., Isupov, M.N. and Littlechild, J.A.
Exeter Biocatalysis Centre, School of Biological
and Chemical Sciences, University of Exeter, UK
P2.20: Bacteriophages as a Source of Nucleic Acid Processing
Enzymes
Hjörleifsdottir, S1; Skirnisdottir, S1; Thorisdottir,
A1,3; Aevarsson, A1; Hreggvidsson, G O1,3; Blöndal, T2,3;
and Kristjansson, J. K1,3
1Prokaria Ltd, Gylfaflöt 5, IS-112, Reykjavik,
Iceland
2deCODE genetics Inc, Sturlugata 8, IS-101, Reykjavik, Iceland
3Institute of Biology, University of Iceland, Grensasvegur 12,
IS-108, Reykjavik, Iceland
P2.21: Biochemical and Regulatory Analysis of Pyruvate
Kinase From Thermococcus kodakaraensis KOD1
Imanaka, H.; Tambalo, R.; Fukui, T.; Atomi, H.; Imanaka,
T.
Department of Synthetic Chemistry and Biological
Chemistry, Graduate School of Engineering, Kyoto University,
Katsura, Nishikyo-ku, Kyoto 615-8510, Japan
P2.22: A Novel Thermostable O-Acetylserine Sulfhydrylase
from Aeropyrum pernix K1
Mino, K; Ishikawa, K.
The Special Division for Human Life Technology,
National Institute of Advanced Industrial Science and Technology
(AIST, Kansai), 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577, Japan
P2.23: A Thermostable L-Aminoacylase from Thermococcus
litoralis
Hollingsworth, E.J.; Isupov, M.; Toogood H. and Littlechild,
J.
Exeter Biocatalysis Centre, School of Biological
and Chemical Sciences, University of Exeter, Exeter, UK.
P2.24: Characterisation of a Novel Compatible Solute
in the Thermophilic Bacterium Petrotoga miotherma
Jorge, C.1; Lamosa, P.1; Santos, H.1
1Instituto de Tecnologia Química e Biológica,
Universidade Nova de Lisboa, Apt 127, 2780-156 Oeiras, Portugal
P2.25: Enzyme Maturation Process of [Ni-Fe] Hydrogenase
from the Hyperthermophilic Archaeon, Thermococcus kodakaraensis
Kanai, T.; Hirao, Y.; Atomi, H.; Imanaka, T.
Department of Synthetic Chemistry and Biological
Chemistry, Graduate School of Engineering,Kyoto University,
Japan, Katsura, Nishikyo-ku, Kyoto 615-8510
P2.26: X-Ray Structure Analysis of Isocitrate Dehydrogenase
from the Hyperthermophile Aeropyrum pernix
Karlström, M.a; Steen, I.b; Tibbelin, G.a; Birkeland,
N.b; Lien, Tb; Ladenstein, R.a
a The Department of Biosciences, The Karolinska
Institute, Sweden
bThe Department of Microbiology, University of Bergen, Norway
P2.27: Analysis of Thermostable Enzymes Isolated from
the Genomic Information of the Hyperthermophilic Archaea
Kawarabayasi, Y.
AIST, Research Center for Glycoscience, Higashi
1-1, Tsukuba, Ibaraki 305-8566 Japan
P2.28: Afung Represents the Principal Uracil DNA Glycosylase
Enzyme Present in the Hyperthermophilic Archaeon Archaeoglobus
fulgidus
Knævelsrud, I1,2; Haugland, GT2; Kazazi, S1; Birkeland,
N-K2; Bjelland, S1
1 School of Science and Technology, Stavanger
University College, Box 8002, N-4068 Stavanger, Norway;
2 Department of Microbiology, University of Bergen, Box 7800,
N- Bergen, Norway.
P2.29: Isolation of Thermostable Variants of Tryptophan
Synthase from Deinococcus radiodurans by DNA Shuffling
in Thermus thermophilus
Koyama, Y.
Institute for Biological Resources and Functions,
National Institute of Advanced Industrial Science and Technology
(AIST), AIST Tsukuba Central 6 , Higashi 1-1-1, Tsukuba, Ibaraki
305-8566, Japan
P2.30: Alpha-Glucosidase Activities from Three Different
Thermococcus
Legin, E ; Caires, N ; Paquis, S ; Conreux, A ; Duchiron,
F
Laboratoire de microbiologie Industrielle, Université
de Reims Champagne Ardenne, Moulin de la Housse BP 1039 Reims
Cedex 2, France
P2.31: A (+)-gamma-Lactamase from the Hyperthermophile
Sulfolobus solfataricus MT4 with Sequence Homology to
the Signature Amidases
Toogood, H.S.1; Line, K.1; Brown, R.C.2; Keene, P.A2;
Taylor, S.J.C2 and Littlechild, J.A.1
1Biocatalysis Centre, School of Biol. And Chem.
Sciences, University of Exeter, UK
2Chirotech Ltd, Cambridge Science Park, Cambridge, UK
P2.32: Crystallization and Preliminary X-Ray Analysis
of a Novel Archaeal Heat Shock Regulator
Liu, W1; Vierke, G2; Thomm, M2; Ladenstein, R*1
1. Center for Structrual Biochemistry, Karolinska
Institute, Novum 14157, Huddinge, Sweden
2. Lehrstuhl Fr Mikrobiologie, Universitt Regensburg, Regensburg
93053, Germany
P2.33: Cloning, Purification and Characterisation of
a Lysophospholipase from Pyrococcus furiosus
Mallett, L,E1; Littlechild, J.A1; Brown, R2; Taylor.
S2.
1Exeter Biocatalysis Centre, Schools of Chemistry
and Biological Sciences, University of Exeter, UK
2Chirotech Technology Ltd., Cambridge, UK (a subsidiary of Dow
Chemical Company)
P2.34: Transport of Mannosylglycerate and Trehalose
in the Thermophilic Bacterium Thermus thermophilus
Sampaio M-M1; Silva Z3; da Costa M S.3; Boos W 2; Santos
H1
1Instituto de Tecnologia Química e Biológica,
Universidade Nova de Lisboa, Oeiras, Portugal;
2Faculty of Biology, University of Konstanz, Konstanz, Germany;
3Dept. Bioquímica, Universidade de Coimbra, Coimbra,
Portugal.
P2.35: Refined Structure of Ornithine Carbamoyltransferase
from the Hyperthermophile Pyrococcus furiosus at 1.87
Å
Massant, J.1; Wouters, J.2; and Glansdorff, N.1
1 Department of Microbiology, Vrije Universiteit
Brussel, Belgium
2 Institut de Recherches J. M. Wiame, Université Libre
de Bruxelles, Belgium
P2.36: Characterization of Geranylgeranylglyceryl Phosphate
Synthase from a Thermoacidophilic Archaeon, Thermoplasma
acidophilum
Nemoto, N.; Oshima, T. and Yamagishi, A
Dep. of Mol. Biol., Tokyo University of Pharmacy
and Life Science
P2.37: Bacterial Adaptation to Extreme pH and Temperature
Olsson, K; Keis, S; Cook G.M.
Department of Microbiology, Otago School of Medical
Sciences, University of Otago, P.O. Box 56, Dunedin, New Zealand
P2.38: A New Intrinsic Thermal Parameter for Enzymes
- The True Temperature Optimum
Peterson, M. E. 1; Eisenthal, R.2; Danson, M. J. 3; Daniel
R. M. 1
1 Department of Biological Sciences, University
of Waikato, Private Bag 3105, Hamilton, New Zealand.
2 Department of Biology and Biochemistry, University of Bath,
Bath, BA2 7AY, UK.
3 Centre for Extremophile Research, Department of Biology and
Biochemistry, University of Bath, Bath, BA2 7AY, U.K.
P2.39: Protein Translocation in an Extreme Thermophile
Pretz, Mg; Albers, Sv; Swaving, J; Garrido V; Driessen,
Ajm;
Department Of Molecular Microbiology, University
Of Groningen, Kerklaan 30. 9751 Nn Haren, The Netherlands
P2.40: Characterization and Crystallization of NADP-Dependent
Glycerol-3-Phosphate Dehydrogenase from Archaeoglobus fulgidus,
a Hyperthermophilic Sulfate-Reducing Archaeon
Sakasegawa, S1, Thauer, RK2; Essen, RO3; Shima, S2
1Asahi Kasei Corporation, Japan
2Max-Planck-Institut for Terrestrial Mikrobiology, Germany
3Department of Chemistry, Philipps-University Marburg, Germany
P2.41: Characterization of Thermostable and Acidiphilic
L-Arabinose Isomerase from Alicyclobacillus acidocaldarius
Lee, S-J; Lee, D-Y; Lee, D-W; Choe, E-A; Kim, S-B; Hong, Y-H
and Pyun, Y-R
Department of Biotechnology and Bioproducts Research
Center, Yonsei University, Seodaemun-Gu, Shinchon-Dong 134,
Seoul 120-749, Korea
P2.42: Dihydroxy-Acid Dehydratase from the Thermoacidophilic
Archaeon Sulfolobus solfataricus
Seonghun Kim, S.1,2 and Sun Bok Lee1,2,3
1School of Environmental Science and Engineering
2Division of Molecular Life and Sciences
3Department of Chemical Engineering, Pohang University of Science
and Technology, Pohang 790-784, Korea
P2.43: Production, Characterization and Applications
of Thermo-Alkalistable Cellulase-Free Xylanase of an Extreme
Thermophile Geobacillus thermoleovorans AP7
Sharma, A.; Archana, A1. ; T. Satyanarayana
Department of Microbiology, University of Delhi
South Campus, Benito Juarez Road, New Delhi-110 0211, India
1Department of Microbiology, Swami Shardhanand College, Alipur,
Delhi-110036, India
P2.44: Thermostability, Cofactor Specificity, Oligomeric
State and Phylogenetic Affiliation of Isocitrate Dehydrogenase
from Aeropyrum pernix, Archaeoglobus fulgidus,
Pyrococcus furiosus and Thermotoga maritima
Steen, IH; Birkeland, N-K
Department of Microbiology, University of Bergen,
Norway
P2.45: Characterization of Isocitrate Dehydrogenase
from Two Moderate Thermophiles, Methylococcus capsulatus
and Thermoplasma acidophilum
Stokke, R.; Birkeland, N.-K.
Department of Microbiology, University of Bergen,
Norway
P2.46: Characterization of an Exo-ß-D-Glucosaminidase
Involved in a Novel Chitinolytic Pathway from the Hyperthermophilic
Archaeon Thermococcus kodakaraensis KOD1
Tanaka T.1; Fukui T.2; Atomi H.2; Fujiwara, S.1; and
Imanaka T.2
1Department of Life Science, School of Science
and Technology, Kwansei Gakuin University, 2-1 Gakuen, Sanda
669-1337, Japan.
2Department of Synthetic Chemistry and Biological Chemistry,
Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku,
Kyoto 615-8510, Japan
P2.47: Thermophilic Nitrilase Enzymes
Waddell, S.J.1, Roberts, S.M.2, Harding, M. 2 and Littlechild
J.A. 1
1School of Biological and Chemical Sciences,
University of Exeter, Exeter, Devon, UK
2Stylacats limited, Cheshire Manufacturing Park, Cheshire, UK
P2.48: 2-Oxoacid: Ferredoxin Oxidoreductases from Aeropyrum
pernix, a Hyperthermophilic and Strictly Aerobic Archaeon
Wakagi, T; Nishizawa, Y; and Fukuda, E.
Department of Biotechnology, The University of
Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
P2.49: Trehalose Metabolism in the Hyperthermophilic
Crenarchaeote Thermoproteus tenax
Zaparty, M.1; Brenner, A.1; Tjaden, B.1; Brinkmann, H.2;Hensel,
R.1; Siebers, B.1
1Department of Microbiology, University Duisburg-Essen,
Essen 45117, Germany
2Département de Biochemie, Université de Montreal,
QC, H3C3J7, Canada
P2.50: The Catalytic Mechanism of Lumazine Synthase
from Hyperthermophile Aquifex aeolicus
Zhang, X.;1,2 Meining, W.;1,2 Cushman, M.;3 Haase, I.;4
Fischer, M.;4 Bacher, A.;4 and Ladenstein, R.1
1Centre for Structural Biochemistry, Novum, Karolinska
Institute, S-14157 Huddinge, Sweden
2Södertörns Högskola, S-14157, Sweden
3Department of Medicinal Chemistry and Molecular Pharmacology,
School of Pharmacy and Pharmacal Sciences, Purdue University,
West Lafayette, Indiana 47907
4 Lehrstuhl für Organische Chemie und Biochemie Technische
Universität München, Lichtenbergstr. 4, D- 85747 Garching,
Federal Republic of Germany
