Thermophiles 2003

Poster Session 1

Tuesday, 16th September

P1.1: Resistance of Isolated Thermophilic Bacillus Phages to High Concentrations of Sodium Azide
Akel, H.1, Abu-Zreig, M.2 and Doker, D.
1 Department of Medical Laboratory Sciences. Hashemite University. Jordan.
2Department of Agricultural Engineering and Technology. Jordan University of Science and Technology. Jordan.

P1.2: An AMP-Dependent DNA Ligase Reaction: An Enzyme Reaction that occurs only at high Temperature
Atomi, H.; Nakatani, M. and Imanaka, T.
Department of Synthetic Chemistry & Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan

P1.3: Cytochrome C555 from the Hyperthermophilic Bacterium Aquifex aeolicus: Heterologous Production and Investigation of the Role of Methionine Residues
Aubert, C., Guerlesquin, F., Bianco, P., Leroy, G., Tron, P and Bruschi, M.
31 chemin Joseph Aiguier 13402 Marseille, Cedex 20, France

P1.4: Isolation of Thermotolerant Dextranases from Soil Bacteria
Bergquist, P.L. 1,2; Finnegan, P.M. 3,4; O'Shea, M.G. 5; Brumbley, S.M.5, and Nevalainen, H. 1,3
1Biotechnology Research Institute, Macquarie University, North Ryde, NSW2109, Australia;
2Department of Molecular Medicine & Pathology, University of Auckland Medical School, Auckland, New Zealand;
3School of Biological Sciences, Macquarie University, North Ryde, New South Wales 2109, Australia;
4School of Plant Biology, University of Western Australia, Crawley, WA, Australia;
5Bureau of Sugar Experiment Stations, PO Box 86, Indooroopilly, Queensland 4068, Australia.

P1.5: A Host-Vector System for Rhodothermus marinus
Bjornsdottir, S.1,2; Thorbjarnardottir, S.H.1; Eggertsson, G.1
1Laboratory of Molecular Genetics, Institute of Biology, University of Iceland
Grensasvegur 12, IS-108 Reykjavik, Iceland
2Prokaria Ltd., Gylfaflot 5, IS-112 Reykjavik, Iceland

P1.6: Hyperthermophilic Metal Resistant Microorganisms from Natural and Contaminated Environments
Blamey, J.M.1; Chavez, P.2; Castro, M.1 and Blamey, L.L.1
1Fundación Biociencia, José Domingo Cañas 2280, Ñuñoa, Santiago, Chile
2Centro De Biotecnología Y Biología Molecular, Facultad Recursos Del Mar, Universidad De Antofagasta, Casilla 170, Chile.

P1.7: Cultured and Uncultured Acetate-Metabolizing Thermophilic Anaerobes
Bonch-Osmolovskaya, E.A.1, Miroshnichenko, M.L.1, Zavarzina, D.G.1, Pimenov, N.V.1, L'Haridon, S.2, Jeanthon, C.2
1Institute of Microbiology, Russian Academy of Sciences, Moscow, Russia
2UMR 6539, Centre National de la Recherche Scientifique & Université de Bretagne Occidentale, Institut Universitaire Européen de la Mer, Plouzané, France

P1.8: Thermotoga maritima-Escherichia coli Chimeric Proteins: Answers about Involvement of the Carboxy-Terminal Domain in DNA Topoisomerase I-Mediated Catalysis
Bouthier de la Tour, C.; Viard, T.; Cossard, R.; and Duguet, M.
Institut de Génétique et Microbiologie. Bât 400. Université Paris sud. 91405. Orsay. France

P1.9: Mutagen, A Free System for Annotating and/or Comparing Genomes
Brügger, K.1; Redder, P.1; Skovgaard, M.2
1Danish Archaea Group, Institute of Molecular Biology, University of Copenhagen, Denmark.
2Center for Biological Sequence Analysis, The Technical University of Denmark, Denmark

P1.10: Online Post-Publication Sulfolobus Genome Resource
Redder, P.; Brügger, K.; Torarinsson, E.; Garrett, R.
Danish Archaea Group, Institute of Molecular Biology, University of Copenhagen, Denmark.

P1.11: Phylogenetic Diversity of Thermophilic Prokaryotes in Se-Reducing Enrichment Cultures from Mono Lake, California
Chernyh, N.A.1; Sokolova, T.G. 1; Subbotina, I.V. 1 ; Madsen, M.S. 2 ; Bonch-Osmolovskaya, E.A.1; Birkeland, N-K. 2
1Institute of Microbiology, Russian Academy of Sciences, Prospekt 60 Let Oktyabrya 7/2, 117312 Moscow, Russia
2 Department of Microbiology, University of Bergen, Jahnebakken 5, P.O. Box 7800, N-5020 Bergen, Norway

P1.12: Using GenomiPhi DNA Amplification Kit for the Representative Amplification of Microbial Metagenomes
Collins, D.J.a; Mamone, J. Anthonya; Blamey, Jenny M.b; Hamilton, Scotta
aAmersham Biosciences, 800 Centennial Avenue, Piscataway, NJ, 08855 USA
bFundacion Cientifica y Cultural Biociencia, Jose Domingo Canas 2280, Nunoa, Santiago

P1.13: Stringent Regulation of Growth-Phase on the Replication of Sulfolobus Helper and Satellite Viruses SSV2 And SSVX
Contursi, P.1,2; Bartolucci, S.2; Rossi, M. 2,3; Garrett, R.A. 1, She, Q.1
1Microbial Genome Centre, Institute of Molecular Biology,Copenhagen University,, Denmark;
2Dipartimento di Chimica Biologica, Universita´ Federico II di Napoli, Italia.
3Istituto di Biochimica delle Proteine, C.N.R. , Napoli, Italia

P1.14: Characterization of Archaeal Thermophilic Chromatin
Fathima Z., Suryanarayana, T..
Department of Biochemistry, University of Hyderabad, Hyderabad 500 046, Andhra Pradesh, India

P1.15: A Mar-Like Transcription Factor in the Archaeon Sulfolobus solfataricus Involved in the Detoxification of Aromatic Aldehydes
Fiorentino, G1.; Ronca, R1; Cannio, R.2; Rossi, M.1,2 ; Bartolucci S1.
1 Dipartimento di Chimica Biologica, Università Federico II, Via Mezzocannone, 16- 80134 Napoli, Italia
2 Istituto di Biochimica delle Proteine, CNR, Via P. Castellino, 111- 80131 Napoli, Italia

P1.16: Retrieval of Amylolytic Enzymes through GENEMININGTM of Environmental DNA
Fridjonsson, O.; Hreggvidsson, G.O.; Skírnisdóttir, S.; Hauksdóttir, S.; Marteinsson, V. TH.; Hobel, C.F. V.; and Kristjánsson, J.K.
Prokaria ltd. Gylfaflöt 5, 112 Reykjavik, Iceland.

P1.17: Analysis of Microbial Communities Associated with Deep North Sea Oil-Wells Using Culture-Dependent and Culture-Independent Strategies
Garshol, F.; Dahle, H.; Madsen, M.; Lien, T.; Birkeland, N.-K.
Department of Microbiology, University of Bergen, Norway

P1.18: Ethanol Tolerance of Thermophilic Anaerobic Ethanol Producers
Georgieva, T.; Ahring, B.K.
The Environmental Microbiology & Biotechnology Research Group, BioCentrum, Technical University of Denmark, Building 227, DK-2800 Lyngby, Denmark

P1.19: Identification, Location, Size And Fit to the "BHB" Splicing Motif Within Intron-Exon Junctions in Archaeal Pre-tRNA's
Grosjean, H.1 and Marck, C.2
(1) Laboratoire d'Enzymologie et Biochimie Structurales, Bat 34, CNRS, Gif-sur-Yvette, France.
(2)Service de Biochimie et de Génétique Moléculaire, Bat 144, CEA/Saclay, Gif-sur-Yvette, France

P1.20: The Pyrococcus abyssi "TrmI" Methyltransferase Displays Region-Specificity Towards its tRNA Substrate and is Stabilized at Extreme Temperatures by Inter-Subunit Disulfide Bridges
Grosjean, H1. , Roovers, M2., Wouters, J.2, Bujnicki, J.M.3, Tricot, C.2, And Droogmans, L2.
1Laboratoire d'Enzymologie et Biochimie Structurales, CNRS, Bat 34, F-91198, Gif-sur-Yvette, France;
2Laboratoire de Microbiologie, Université Libre de Bruxelles, Institut de Recherches Microbiologiques J-M Wiame, 1, av. E. Gryson, B-1070 Bruxelles, Belgium;
3Bioinformatics Laboratory, International Institute of Molecular & Cell Biology, ul. ks. Trojdena 4, 02-109 Warsaw, Poland.

P1.21: Novel Viruses from Hot Acidic Springs in Pozzuoli, Italy
Häring, M.; Rachel, R.; and Prangishvili, D.
Department of Microbiology, Archaea Centre, University of Regensburg, Universitätstr. 31,
D-93053 Regensburg, Germany

P1.22: Complete Genome Analysis of the Hyperthermophilic Archaeon, Thermococcus kodakaraensis KOD1: General Features And Horizontal Gene Transfer
Imanaka, T.1); Fukui, T.1); Kanai, T.1); Fujiwara, S.2); Atomi, H.1)
1) Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan
2) Department of Life Science School of Science and Technology, Kwansei Gakuin University, 2-1 Gakuen, Sanda 669-1337, Japan

P1.22a: Complete Genome Analysis of the Hyperthermophilic Archaeon, Thermococcus kodakaraensis KOD1: Comparison of Genomes at the Order Level
Fukui, T.1); Kanai, T.1); Fujiwara, S.2); Atomi, H.1); Imanaka, T.1)
1) Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan
2) Department of Life Science School of Science and Technology, Kwansei Gakuin University, 2-1 Gakuen, Sanda 669-1337, Japan

P1.23: Innovative Technology for Isolation of New Genotypes from Hot Environments
Ishøy, T.; Kvist, T.; Westermann, P.; Ahring, B. K.
Environmental Microbiology and Biotechnology Group (EMB), BioCentrum-DTU, Technical University of Denmark, Denmark

P1.24: Thermophile-Specific Proteins: The Gene Product of Aq_1292 From Aquifex aeolicus is an NTPase
Kaufmann, M1; Daumke, O2; Howe, R1; Klinger, C1; Roßbach, M1; Wittinghofer, A2
1Institute for Neurobiochemistry, The Protein Chemistry Group, Witten/Herdecke University, Germany
2Max-Planck Institute of Molecular Physiology, Department I - Structural Biology, Germany

P1.25: Screening for New Anaerobic Thermophilic Prokaryotes with Hydrolytic Activities
Kublanov, I.V., Subbotina, I.V., Chernyh, N.A., Kostrikina, N.A., Turova, T.P. Bonch-Osmolovskaya, E.A.
Institute of Microbiology, Russian Academy of Sciences, Russia

P1.26: alpha-Glucosidases from Thermotoga Species: Biochemical Properties and Structure-Function Relationships
Liebl, W.
Institute of Microbiology and Genetics, Georg-August-University, Goettingen, Germany

P1.27: Comparative Genomics and Experimental Studies on Uracil Recycling in Three Pyrococcus Species
Lucas, S., Leduc, D., Matsunaga, F., Filee, J., Forterre, P. and Myllykallio, H.
Institut de Génétique et Microbiologie, Université de Paris-Sud, 91405 Orsay, France

P1.28: Thermus sp. Strain KW11 and Cloning of the Gene of DNA Polymerase I
Matsukawa, H.; Kurosawa, N.
Department of Environmental Engineering for Symbiosis, Soka University, Japan

P1.29: DNA Replication, DNA Repair and Cell Cycle Regulation in Pyrococcus abyssi
Matsunaga, F1; Jolivet, E2; Prieur, D2; Myllykallio, H1 and Forterre, P1
1 Institut de Génétique et Microbiologie, Université Paris-Sud, France
2 Institut Universitaire Européen de la Mer, Université de Bretagne occidentale, France

P1.30: Nautiliales ORD. NOV. - A New Order Within the Class Epsilonproteobacteria
Miroshnichenko M. L.1, L'Haridon S.2, Schumann P.3, Spring S.3, Bonch-Osmolovskaya E. A.1, Jeanthon C.2, Stackebrandt E.3
1Institute of Microbiology, Russian Academy of Sciences, Prospect 60-letiya Oktyabrya 7/2, 117811 Moscow, Russia
2UMR 6539, Centre National de la Recherche Scientifique & Université de Bretagne Occidentale, Institut Universitaire Européen de la Mer, 29280 Plouzané, France
3German Collection of Microorganisms and Cell Cultures DSMZ, Mascheroder Weg 1b, 38124 Braunschweig, Germany

P1.31: Isolation and Characterization of Extreme Thermophile YMO81
Moriya, T1; Takahashi, N1; Yokobori, S1; Yamagishi, A1; Oshima T1
School of Life Science1, University of Pharmacy and Life Science Japan 1432-1 Horinouchi Hachiouji-shi Tokyo Japan

P1.32: Does Microbial Activity Confer Structure (Biosignatures) to Siliceous Hot-Spring Deposits?
Neal, A. L.1,2; Kyle, J.3; Romanek, C. S.2,3; Schroeder, P.A.3
1 Department of Microbiology, University of Georgia, Athens, GA, U.S.A.
2 Savannah River Ecology Laboratory, P.O. Drawer E, Aiken, SC, U.S.A.
3 Department of Geology, University of Georgia, Athens, GA, U.S.A.

P1.33: SS-LRBP, An LRP-like Transcriptional Regulator from the Hyperthermoacidophilic Crenarchaeon Sulfolobus solfataricus P2, Binds Cooperatively to Three Targets in its own Promoter Region
Peeters, E.1, Gigot, D.2, Thia-Toong, T.-L.1 & Charlier, D. 1
1Erfelijkheidsleer en Microbiologie, Vrije Universiteit Brussel, Building E, Pleinlaan 2, B-1050 Brussels, Belgium. 2Microbiologie, Université Libre de Bruxelles and Institut de Recherches Microbiologiques J.-M. Wiame, E. Grysonlaan 1, B-1070 Brussels, Belgium

P1.34: Desulfurococcus fermentans sp. Nov., A New Hyperthermophilic Archaeon from Kamchatka Hot Spring
Perevalova, A.A.1; Svetlichny, V.A.1; Chernyh, N.A.1; Kostrikina, N.A.1; Turova, T.P.1; Kuznetsov, B.B.2; Bonch-Osmolovskaya, E.A1.
1Institute of Microbiology, Russian Academy of Sciences, Russia
2Bioengineering Center, Russian Academy of Sciences, Russia

P1.35: Microbial Diversity Obtained by Enrichment Culture in Bioreactor of Thermophilic Microorganisms from Hydrothermal Samples of the Medio-Atlantic Ridge
Postec, A1 ; Urios, L 1; Ollivier, B 2; Querellou, J 1 and Godfroy, A1.
1 Laboratory of Microbiology and Biotechnology of Extremophiles, Drv/Vp Ifremer, Brest Center, BP70, 29280 Plouzane, France.
2 Laboratory of Microbiology, Institute of Research and Development, University of Provence and Mediterranée, ESIL case 925, 163 Avenue de Luminy, 13288 Marseille cedex 9, France

P1.36: Properties of Pigmented Thermophilic Bacteria Isolated from Carbonate Hot Springs in Colorado, Yellowstone and India
Ramaley, R.1; Haas, E. 2; and Furlong, B.3
1Dept. of Biochemistry and Molecular Biology, University of Nebraska Medical Center, USA
2Sequencing Analysis Core Facility, University of Nebraska Medical Center, USA
3School of Nursing, Creighton University, USA

P1.37: Biodiversity and Biodegradation Ability of Thermophilic Fungi
Reddy, S.M.; Girisham, S. and Sujatha, E.
Department of Botany & Microbiology, Kakatiya University, Warangal 506 009, (A.P.)

P1.38: The Multi-Layered Structure of DPS with a Novel Di-nuclear Ferroxidase Center
Ren, B.1; Tibbelin, G. 1; Kajino, T. 2; Asami, O.2; and Ladenstein, R.1
1Center for Structural Biochemistry, Karolinska Institutet, NOVUM, S-141 57 Huddinge, Sweden
2Biotechnology Division, Toyota Central R&D Laboratories Inc., Nagakute, Aichi 480-11, Japan

P1.39: Sulfate Metabolism Across a Thermal Gradient
Roychoudhury, A. N.
Department of Geological Sciences, University of Cape Town, Rondebosch, South Africa 7700

P1.40: Development of Polyclonal Antibodies against MT13 Strain of Sulfobacillus thermosulfidooxidans and Determination of their Specificity
Saleem, M.; Anwar, M. A; Ghauri, M. A; Akhtar, K; Rehman, M; Hafeez, F. Y. and Khalid, A. M.
Bioprocess Technology Division, National Institute for Biotechnology and Genetic Engineering, P. O. Box 577, Jhang Road, Faisalabad, Pakistan

P1.41: Construction of a Gene Disruption System by Homologous Recombination in the Hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1
Sato, T.; Fukui, T.; Atomi, H. and Imanaka, T.*
Department of Synthetic Chemistry and Biological Chemistry,1
Graduate School Engineering, Kyoto University,2
Katsura, Nishikyo-ku, Kyoto 615-8510, Japan3

P1.42: A Novel thermostable Neoschizomer of SmaI From a Thermus sp. Isolated from Geothermal Northern Himalayan Region of India
Sharma, P.1; Parashar, V1; Capalash, N2
Department of 1Microbiology and 2Biotechnology, Panjab University, Chandigarh 160014, India

P1.43: Subunit Interaction and Regulation of Activity Through the Terminal Domains of the Family D DNA Polymerase from Pyrococcus horikoshii
Shen, Y.; Tang, X.-F.; Matsui, E.; Matsui, I.
Biological Information Research Center, National Institute of Advanced Science and Technology, Tsukuba, Ibaraki 305, Japan

P1.44: Structural Investigations of a Na(+)/(H+) Antiporter from Methanococcus jannaschii
Smits, S., Kumar, V., Rhinow, D., and Kühlbrandt, W.
Max Planck Institute for Biophysics, Marie-Curie -Strasse 15, 60439 Frankfurt am Main

P1.45: Novel Representative of the Genus of Thermoanaerobacter Capable of Agarose Hydrolysis
Subbotina, I.V.; Chernyh, N.A.; Lebedinskii, A.V.; Bonch-Osmolovskaya, E.A.
Institute of Microbiology, Russian Academy of Sciences, 117312 Moscow, Russia

P1.46: DNA Aggregation by an Archaeal DNA Binding Protein. SAC10B and its Novel DNA Nicking Activity
Suryanarayana, T.; Jaya, K.S. and Sreenivas, K.
Department of Biochemistry, University of Hyderabad, Hyderbad -500046, India

P1.47: Rooting the Tree of Life with tRNA Genes
Tong, K.-L.; Xue, H.; Wong, J.T.-F.
Department of Biochemistry and Applied Genomics Center, Hong Kong University of Science and Technology, Hong Kong

P1.48: Tepidibacter formicigenes sp. Nov., A Novel Spore-Forming Bacterium Isolated from a Mid-Atlantic Ridge Hydrothermal Vent
Urios, L; Cueff, V; Pignet, P and Barbier, G.
Laboratoire de Microbiologie et de Biotechnologie des Extrêmophiles, Département de Valorisation des Produits, Ifremer, Centre de Brest, BP 70, 29280 Plouzané, France

P1.49: Analysis of Bacterial Communities in Solid-Waste Composting System
Watanabe, K.; Kurosawa, N.
Department of Environmental Engineering, Soka University, Japan

P1.50: Experimental Test: If the Common Ancestor was a Hyperthermophile?
Yamagishi, A.; Watanabe, K.; Iwabata, H.; Ohkuri, T.; Yokobori, S.
Department of Molecular Biology, Tokyo University of Pharmacy and Life Science, Japan